In the current agricultural context, biological control is advancing, and entomopathogenic fungi play a crucial role in integrated pest management. It is necessary to cultivate these entomopathogenic fungi on a large scale for their use in pest control. Fungi rely on essential nutrients for their growth, which can be supplemented using different culture media. These media provide the fungi with carbohydrates, minerals and moisture. Different media support the growth of fungi. In the particular study, the effects of three media (PDA, SDAY, and SMAY) were assessed on eight fungal isolates; including four of Beauveria bassiana and one isolate each of Penicillium simplicissimum, Clonostachys rosea, Purpureocillium lilacinum, and Talaromyces pinophilus. SDAY was found to be the most favourable medium for the growth of all fungi, except for one isolate of Beauveria bassiana, which exhibited the highest response in PDA.