The proteome profile of bodily fluids can be influenced by blood contamination during the collecting of samples. Visual inspection and counting of red blood cells in the sample are commonly used in proteomics investigations to rule out tainted samples; particular blood-derived protein analysis is less common. We devised a rapid and sensitive approach for detecting blood contamination in fundamental biological fluids based on a unique blood-derived protein, haemoglobin detection by ultraviolet visible spectrometry, to bridge the gap. The reference approach, UV spectrometry, was found to be less sensitive. The absorption and scattering properties of particle suspensions are represented in the UV spectrum. This data can be utilised to decipher the spectrum in terms of particle size distribution, particle shape, and chemical composition of the sample material. Because of the existence of various chromospheres found in proteins, strong plasma absorption in the UV area can offer information about these proteins from the plasma. The current method has several advantages, including being rapid, effective, and sensitive, requiring a small sample size, and being able to detect blood contamination in a range of physiological fluids collected for proteomics study.