The aim of this research is to evaluate the essential amino acid contents of some underutilized wild edible fruits of different districts of Odisha. An essential amino acid composition was evaluated using UV-VIS Spectrophotometer. As essential amino acids cannot be synthesized in the body, they must be obtained through diet. The adequate amounts of essential amino acid contents are required for the normal functioning and development of physiological processes. Antidesma acidum, Flacourtia indica, and Walsura trifoliate are predominantly contains methionine, tryptophan, isoleucine, valine, leucine and histidine. Antidesma acidum come out to be a good source of methionine 1241.7±0.92 mg/100g dw and tryptophan 1589.7±1.34 mg/100g dw content. Methionine acts as antioxidants and protects from ionizing radiation, detoxifying heavy metals and paracetamol poisoning.