Background: Determination of sex is very important in forensic medicine. Barr bodies are unique chromatin structures formed in nuclei of the mammalian females as a means of sex chromosome dosage compensation. Special stains on buccal scrapes for detection of Barr bodies for sex determination Hence this, preliminary study was aimed to identify gender using buccal Barr bodies to compare the staining efficacy of papanicolau, aceto orcein stain and feulgen stain.