The present work aimed to evaluated the antifungal and antioxidant potential of geranium oil extracted by different methods simultaneously. The geranium oil was extracted by Clevenger method, solvent extraction by soxhlet apparatus and microwave assisted hydrodistillation method. The percent yield oil obtained by microwave assisted method was found to be more than clevenger method. Solvent extraction method produced less amount of oil. The samples were further studied for their antifungal action, which was found better in oil obtained by clevenger method. The same samples when analyzed for their antioxidant potential, showed comparatively higher value. The study also involved development and validation of HPLC method for determination of geraniol constituent in oil samples. It was concluded that oil extracted through clevenger method exhibited better antifungal and antioxidant action. The developed and validated method for evaluation of geraniol was found to be precise and accurate and robust, also utilized in routine analysis of raw material.