Background: Hospital-acquired infections are considered one of the most serious problems in the world. The problem of infection prevention and control in hospitals becomes more serious, especially with the scarcity of resources and materials; also, the lack of supportive policies and direction can exaggerate the situation. The CDC recommended that educating healthcare workers regarding infection control measures is the highest priority to prevent and control nosocomial infections. Aim: The study aimed to assess the healthcare workers' practices in the operating rooms in Al Noor Specialist Hospital in King Saudia Arabia (KSA). Research Design: A cross-sectional descriptive survey design. Subject: participants in the study included a convenience sample of healthcare workers (n=100) who worked in the OR at the time of data collection. Tool: a self-administered questionnaire for the HCWs' knowledge, attitudes, training & education, and practices for the OR setting. Results: The study results show that the majority of HCWs know about IPC practices and their role in reducing nosocomial infections. Also, the majority of HCWs have good attitudes towards IPC practices. These results are considered a good chance for improvement and development in this context. Recommendations: Training courses and education sessions regarding IPC should be implemented for all HCWs to increase their awareness of this issue.