Autografts remain the standard of care in the treatment of large cutaneous defects caused by burns, disease (e.g., tumor excision, diabetic foot ulcers, decubitus ulcers, venous leg ulcers), and traumatic injury. The harvest of split-thickness skin graft (STSG) consisting of epidermal and dermal tissue creates a partial-thickness wound that involves pain management, wound care, and healing time. The photobiochemical effects observed at the cellular level result from the interaction of electromagnetic radiation with cells and tissues. Therapeutic or low-power lasers are used to accelerate tissue repair processes due to their biomodulatory effects on cells. They activate or inhibit physiological, biochemical and metabolic processes through photophysical or photochemical effects. The benefits of PBM applied to wounds are well-described in the literature. The findings include anti-inflammatory action, improved tissue organization, the acceleration of angiogenesis, the stimulation of the chemotaxis of leukocytes, and a reduction in the size of the donor site in animals. Although clinical studies have reported significant effects of PBM on wounds, only one study was found investigating the effect of this method on the donor area of a skin graft. In the study, the authors divided the participants into two groups, both with the same dressing (Mepitel® membrane and petroleum jelly). A group was also associated with PBM. They proved that the reduction was significantly greater in the group with dressing and PBM