Hepatitis is an inflammation of the liver cells that is caused by a variety of infectious viruses. Aim: This study aimed to assess of mothers’ knowledge and practices regarding care of their children with hepatitis viruses. Design: A descriptive design was utilized in this study. Sampling: A purposive sample comprised of 60 mothers having children with Hepatitis Viruses was selected to conduct this study. Setting: This study was conducted at blood diseases outpatient clinic in University hospital affiliated to Beni Sueif University Hospital. Sample: A purposive sample were included in the study. Tools: Two tools were used in this study Tool (1): A structured questionnaire to assess mothers’ knowledge regarding Hepatitis Viruses. Tool (2): Practice checklists to assess mothers’ reported practices regarding care of their children with Hepatitis Viruses. Results: Findings of this study showed that,60% of studied mothers had unsatisfactory level of knowledge , and their total practices were inadequate also there was a highly statistically significant relation between total knowledge and practices of mothers and their characteristics particularly educational level and occupation p≤0.05. Conclusion: The studied mothers had unsatisfactory level of knowledge and inadequate practices regarding care of their children with Hepatitis Viruses, there was a statistically significant difference between studied mothers’ total level of knowledge and reported practices and their characteristics. Recommendations: Continuous educational program for mothers about hepatitis viruses are required to increase their awareness and performance regarding care of their children with hepatitis viruses.