This research focused on the impacts of silica nanoparticles (NPs), nano glass particles (NG), and Capsicum annuum plants with an unnamed chemical to study their response to different treatments. To gauge the physiological reactions of the plants, growth metrics, chlorophyll concentrations, enzyme activity, moisture content, and fresh/dry weights were measured. According to the findings, silica NPs, NG, and the unknown compound all had detrimental impacts on plant development and physiology as their concentrations rose. With increasing treatment concentrations, biomass accumulation, root, and shoot lengths, and chlorophyll levels were all reduced. The results of the therapy were clearly shown in the enzyme activity test, which measured enzyme activity. These results urge for more research into the underlying processes and highlight the possible negative impacts of these chemicals on plant health and production