Introduction: Gastrointestinal anomalies are structural defects and incomplete or abnormal development of digestive organs can cause blockages, vomiting and problems with bowel movements. Nurses identify problems and implement nursing care for children undergoing gastrointestinal surgeries. The study was aimed at assessing nurses' knowledge and practices towards caring of children undergoing gastrointestinal. Design: A descriptive design was utilized in conducting the study. Settings: This study was conducted at surgical departments of a pediatric hospital at Makkah hospitals, Subjects: Purposive sample composed of all (60) available nurses in the previously mentioned settings. Tools, A questionnaire sheet, and observation checklists. Results: the study results illustrated that, less than two-thirds of the studied nurses had satisfactory total knowledge, while more than two-thirds of them had competent actual total practice regarding care of children undergoing gastrointestinal surgeries. Conclusion: Nurses’ knowledge and practice regarding care of children undergoing gastrointestinal surgeries were satisfactory knowledge and competent practice by more of them half of the nurses. Recommendation: More continuous monitoring of research to assess nurses’ knowledge and practice regarding care of children undergoing gastrointestinal surgeries.