Quality in electronic learning is critical to ensuring beneficial nursing student experiences in electronic courses as it is based on the perspective of students. Aim was to assess nursing students' knowledge and perspective about quality in electronic learning. Methods: A descriptive research design was used. All third and fourth year nursing students in the academic year 2021-2022 students at faculty of nursing; Fayoum University (n=821). Two tools were used for collecting data I- Nursing students’ knowledge about quality in electronic learning questionnaire and II- Nursing students' perspective questionnaire. Results: Regarding total level of knowledge, less than half of nursing students 41% had correct knowledge about quality in electronic learning, whereas more than half of them 59% had incorrect knowledge about quality in electronic learning. Regarding perspective about quality in electronic learning, more than two thirds 69.3% of nursing students had a positive perspective about quality in electronic learning. While less than one quarter 21.33% of them had a negative perspective about quality in electronic learning with. Additionally that there was a statistical highly significant association between total knowledge scores and total perspective scores among nursing students. Conclusion: The study findings concluded that more than two fifth of the study sample had satisfactory level of knowledge and two thirds of nursing students had positive perspective about quality in electronic learning. Recommendation: •Training programs about electronic learning and quality in e-learning should be conducted to improve nursing educators and nursing students' knowledge, skills, and attitude toward e-learning