In the pharmaceutical industry, tamarind is often utilized as a mucoadhesive polymer, a naturally occurring polysaccharide that is derived from tamarind seed. However, tamarind's organic application in drug delivery is limited but, its functionalization has shown to enhance its functionality. The contemporary paintings were created with the intention of evaluating the tamarind's observed oral toxicity. IR and mass spectroscopy have almost completely evaluated the functionalized and unmodified tamarind. Results: The experiments confirmed and predicted that sulphonation and thionation of pure tamarind results in effective functionalization. The functionalized and pure tamarind's oral toxicity was tested in albino Wistar rats. Data obtained revealed a notable distinction between Hb and platelet levels. When compared to natural tamarind, functionalized tamarind did not exhibit any acute oral toxicity. Conclusions: The functionalized tamarind is a unique, risk-free, and efficient carrier for the oral administration of medicines.