Purpose: The goal of the present investigation was to determine how ultraviolet (UV) radiation affected polyether ether ketone's (PEEK) osteoblastic activity. Materials And Procedures; PEEK disc samples number thirty. The samples were divided into two groups: PEEK with no therapy (group I; n = 15) and PEEK modified by UV radiation (group II; n = 15). Human osteoblastic sarcoma cells were used as the experimental group's seeding material. The specimens were incubated for 48 hours at 37°C with a 1% relative humidity in a humid environment. 2.5% glutaraldehyde was used to attach the seeded cells to the coverslips after 48 hours. SEM images of the discs were taken in order to assess osteoblastic cell adherence and colony development on the PEEK discs. Results: In contrast to PEEK samples without therapy, UV-treated PEEK samples showed observable osteoblast adherence. The PEEK samples that had not been treated had fewer colonies and less widely dispersed cells. UVmodified PEEK displayed more observable osteoblast cells dispersed throughout the sample. Contrary to group I, the cell adhesion was improved. After using Fisher's exact test to analyze the data, the difference between the test and control groups was statistically significant. In conclusion, UV-modified PEEK had more pronounced osteoblast cells dispersed throughout the sample. When compared to samples that had not been treated with UV, the adherence of the cells was improved.