Polyherbal formulation consisting of plant ingredients of Brahmi (Bacopa monniera), Yastimadhu (Glycyrrhiza glabra), Tagar (Valeriana wallechii) and Ashwagandha (Withania somnifera). Objective: The present study was undertaken to investigate the effects of polyherbal formulation on learning and memory in experimental animals. Methods: Elevated plus-maze (EPM) and passive avoidance paradigm were employed to test learning and memory. Scopolamine (1mg/kg i.p.) and diazepam (1mg/kg i.p.) were used as interoceptive (stimulus inside the body) behaviour model. Three doses (5, 10 and 15 ml/kg p.o.) of polyherbal formulation were administered for 7-14 successive days in separate groups of animals. Results: Elevated plus-maze (EPM) and passive avoidance paradigm model results show that dose of 15 ml/kg of polyherbal formulation significantly improved learning and memory of mice. Furthermore, this dose significantly reversed the amnesia induced by diazepam (1mg/kg i.p.) and scopolamine (1mg/kg i.p.). Since scopolamine-induced amnesia was reversed by polyherbal formulation, it is possible that the beneficial effect on learning and memory was due to facilitation of cholinergic-transmission in mouse brain, also diazepam which is a GABA mimetic agent induces memory impairment and the subsequent inhibition of diazepam induced amnesia by polyherbal formulation may be due to inhibition of GABA-B receptors has been found to facilitate learning and memory. Conclusion: In the present investigation, polyherbal formulation (15ml/kg, p.o.) has shown promise as a memory enhancing agent in experimental animals in all the laboratory models employed