As the demand for sustainable agriculture grows, the use of non-toxic chemicals to manage insect pests is on the rise. Entompathogenic fungi (EPF) like Beauveria and Metarhizium have been extensively studied and their growth has been well optimized in commonly known growth media. However, for novel EPF species, the optimization of growth media is still limited. Fungi rely on essential nutrients for their development, and various culture media can be used to supplement these nutrients, providing carbohydrates, minerals and moisture to support their growth. In this study, we tested six different growth media to assess their efficacy for cultivating some well-known as well as potential EPFs such as Beauvaria bassiana, Clonostachys rosea, Talaromyces muroii and Fusarium keratoplasticum. The results revealed that the fungi showed robust growth on PDA and CDA, while CWM supported the least growth of the EPF species.