Background: Health care staffs working in the blood banks are constantly exposed to microorganisms. Many of which can cause serious or even lethal infections. Lab technician in particular are often exposed to various infections during the course of carrying out their lab activities. Therefore lab activities should have sound knowledge and strict adherence to infection control practice and transfusion services are at risk of exposure to pathogenic organisms in blood. While transfusion- transmissible diseases, including AIDS and viral hepatitis, continue to spread especially in developing countries, the issue of safeguarding the world's blood supply is of paramount importance and thus its blood supply has the potential to affect the global community. In recent years, Saudi Arabia blood centres have tried to improve the nation's blood safety. Although substantial progress has already been made, many daunting difficulties remain. Aim of study: To Assessment of Infection control precaution in blood banks in Makkah Al-Mukarramah Saudi Arabia 2022. Methods: This cross sectional study included (200) health care professionals in Makkah City at Saudi Arabia 2022. (doctors, nurses, lab workers) from primary healthcare (PHC) centers an self-administrated questionnaire was constructed by the researcher and was used for data collection. Divided in to 3 parts and contains items i.e., socio‑demographic characteristics, questions about infection control precaution regarding infection control in blood banks. Results: there were 200 participants, the majority regarding the degree of availability of needs for precautionary measures the majority of participant answer available were (66.0%) a significant relation were P-value=0.001 X2 99.04. The degree of application of precautionary measures the majority of participant answer The procedures are implemented with high quality were (81.0%).Conclusion: performance toward infection control in blood bank it can be concluded that in spite of having good practice level regarding infection control in blood bank , health care worker had fair Infection control precaution in blood bank not bad, but must be updating knowledge and practice of health care worker through continuing in-service educational programs.