Coronavirus disease (COVID-19), is a zoonotic beta-coronavirus that was first reported and became diffuse within Wuhan in China, Then the disease rapidly spread throughout China and worldwide, becoming a global health emergency. Objective: This study aimed to assess levels of anxiety, depression and quality of life among nurses at Fayoum isolation hospital during pandemic Covid – 19. Methods: A descriptive research design was used to conduct this study. The study was conducted at Fayoum Hospital for Health Insurance. Purposive sample was used with sample size (150) nurses in Fayoum health insurance. Tools: Four tools were used for data collection; 1st tool: Socio – demographic sheet, 2nd tool: Zung Self-Rating Anxiety Scale, 3rd tool: Zung Self-Rating Depression Scale, 4th tool: Professional Quality of Life Scale (PROQOL). Results: The mean age of studied nurses was 35.13±6.49 years, the majority (97.3%) of sample were females, more than one third (34%) have severe depression, nearly one third (29.3%) have extreme anxiety and more than two fifths (42.7%) of studied sample were have low quality of life. Conclusion: There were highly statistically significant differences between total depression, anxiety and QOL with age level of education and years of experience. While there were statistically significant differences between total depression, anxiety and QOL with gender. Also, there were highly statistically significant and Positive correlation between total depression and total anxiety. Also, there is a highly statistically significant and negative correlation between total QOL with depression and anxiety.