Neonatal sepsis is a clinical syndrome characterized by signs and symptoms of infection with evidence of bacteremia in the first month of life. Neonatal sepsis is broadly divided into early-onset sepsis (EOS), defined as sepsis within the first 72 hours after birth, and late-onset sepsis (LOS), defined as sepsis clinically evident after 72 hours of birth. Blood culture is the gold standard for the diagnosis of neonatal sepsis; however, culture-negative sepsis is of great concern. This study was conducted after obtaining ethical clearance certificates from the institutional ethics committee. This case-cohort study had a sample size of 56 neonates. The sex, gestational age, birth weight, and day of life of the neonates were noted.