Experiments were conducted on Aspergillus flavus inoculated groundnut to evaluate the effect of cold plasma on the mitigation of aflatoxin. A dielectric barrier discharge type of cold plasma equipment was used to generate plasma at voltage levels of 12, 15 and 18 kV. The exposure time of groundnut samples to plasma was kept at 5, 10 and 15 min in the trials. Argon gas at a flowrate of 15 L/min was used for cold plasma generation. There was a significant reduction of AFB1 in groundnut when treated with cold plasma. The voltage level and exposure time were found to affect the level of aflatoxin degradation. A maximum of 60% reduction of AFB1 was observed at a voltage of 18 kV and exposure time of 15 min. It is concluded that cold plasma treatment can be an effective tool for mitigation of aflatoxin in groundnut.