Introduction: Blood transfusion audits are extremely important in the health-care system to asses & educate those requesting blood components. It provides documentation of the measures taken for improvement and their results, as well as the safe and rational use of blood products. Materials and Methods: This was a cross sectional study, conducted in the blood centre of Vinayaka Missions Kirupananda Variyar Medical College and Hospitals, Salem over a period of 6 months. A total number of 1020 forms were included, requested from July 2022 to December 2022. Name, gender, age, ward, bed number, provisional diagnosis or indication of transfusion, history of blood transfusion, requested blood component, number of units required, nature of request (urgent, immediate, when convenient), date and time of when required, and requesting doctor's name and signature were included as required parameters. The results obtained were analyzed using SPSS version 24. Results: Among 1020 blood requisition forms audited, the name was filled out in all 1020 (100%), age 744 (73%), gender 561 (55%), ward 958 (94%), bed number 51 (5%), provisional diagnosis 183 (18%), history of previous transfusion 173 (17%), hemoglobin 663 (65%), blood grouping 673 (66%), Rh typing 765 (75%), Authorized by 816 (80%), Specimen sent by 805 (79%), Sign of medical officer 693 (68%),type of blood component 714 (70%), Number of units 102 (10%) and Date and time of requirement was filled in 92 (9%) forms. Conclusion: From this study, we can infer that audit of all blood requisition forms at the point of receiving is vital in determining blood requisition errors. Our study demonstrates, improvement in current blood transfusion practices through CME & training of staff is necessary to increase amenability regarding sending appropriate filled blood requisition forms & samples to blood centre.