Aim :Students of Higher Education institutes need to know aboutpreventing methods ofthalassemia. This study targeted to investigate the awareness and attitude of non-medical students at higher education institutesabout premarital counseling and factors related to thalassemia. Methodology : This is a descriptive- cross-sectional research. The samples were 519 non-medical students of Iranshahrhigh education institutes. The gathering data tool was Hajian's standard questionnaire. The data were analyzed using one-sample t-test, Pearson's correlation test and two-way analysis of variance tests through SPSS22. Results:The awareness and attitude of non-medical students of Iranshahr higher education centers about premarital counseling and factors related to thalassemia disease was at the optimal level