To study the knowledge and awareness of the women health care workers about HPV and HPV vaccine to establish the need of more aggressive educational programmes. Method- A descriptive cross sectional study was conducted at Saraswati medical College, Unnao among 250 women health care workers. A structured questionnaire was used to gather information regarding sociodemographic characteristics, awareness and knowledge toward cervical cancer and its screening . Results were assessed depending upon their scores. Result- Out of 244 participants, 66.8% (n = 163) of them had previously heard about the disease. 35.2 % of the participants answered “don’t know” when asked if HPV is sexually transmitted. 34.0 % (n = 83) did not know whether HPV causes cancer. Only 10.4 % (n = 26) answered correctly that HPV infection occurs in both men and women. Although 62.7%(n=153) were aware of the fact that cervical cancer is very much responsible for the cancer related deaths in females, still only 48% of them knew about the screening facility available nearby. Conclusion- The knowledge of the participants in the our study with respect to HPV and cervical cancer is not very high and their awareness related to HPV vaccination is relatively low. This study suggests that there is a need of advocating more aggressive educational and promotional activities on HPV and HPV vaccination at health care facilities as well as in the community.