Baker’s yeast (BY) efficient catalyst for organic transformation. A well planned, budget Reducing, eco-friendly deals will created for the Knoevenagel condensation reaction of benzaldehyde with active methylene in nonaqueous solvent taking very cheaply, easily available biocatalyst, baker yeast. The baker yeast better results in organic solvent, methanol with no any changes in catalytic power. In this reaction malononitrile contain highly active methylene group therefore selected for this reaction. Different derivatives of benzaldehyde with active methylene group of malononitrile used for this reaction. Synthesize product will be confirmed by using spectral analysis.