The barriers to collaboration include differences in professional training and scope of practice, lack of interprofessional communication, hierarchical structures within healthcare settings, time constraints, and limited resources. Additionally, resistance to change, competing priorities, and organizational culture may impede effective teamwork between pharmacists and nurses in antibiotic stewardship programs. On the other hand, several facilitators can enhance collaboration, such as mutual respect, shared goals, interprofessional education, effective communication strategies, leadership support, and the use of technology for information sharing. Understanding these barriers and facilitators is essential for developing strategies to improve pharmacist-nurse collaboration in antibiotic stewardship programs. By addressing these challenges and leveraging facilitators, healthcare organizations can enhance the effectiveness of their antibiotic stewardship initiatives and contribute to the global efforts to combat antibiotic resistance. This review article explores the barriers and facilitators to pharmacist-nurse collaboration in the implementation of antibiotic stewardship programs within public health campaigns. Antibiotic resistance is a significant global health concern, and collaborative efforts between pharmacists and nurses play a crucial role in promoting appropriate antibiotic use and combating resistance. However, various challenges hinder effective collaboration between these two healthcare professionals. This review synthesizes existing literature to identify key barriers and facilitators that influence pharmacist-nurse collaboration in antibiotic stewardship initiatives.