This review article explores the challenges and barriers faced by nurses in low-resource healthcare settings when implementing evidence-based sterilization protocols. Sterilization is a critical aspect of healthcare that ensures the safety of patients by preventing infections and the spread of diseases. However, inadequate resources, limited training, and a lack of awareness about evidence-based practices often hinder nurses from effectively implementing sterilization protocols. This paper synthesizes existing literature to identify common barriers and proposes strategies to overcome these challenges. By addressing these barriers, healthcare facilities can enhance patient safety and improve the quality of care provided in low-resource settings.