The nanoparticle contains Ketoprofen and Betamethasone used for decreasing inflammation and simultaneously inhibits the transcription of Interleukin-12b (Il12b) in microphages. The purpose of this research is to develop mucoadhesive formulation of chitosan and sodium alginate nanoparticle of betamethasone and ketoprofen using the ionotropic gelation process. Prepare nanoparticles were proceeding for the physicochemical characterization like zeta potential, Particle size, morphology, entrapment efficiency, in-vitro release, FTIR and stability. Further, the anti-inflammation activity was confirmed by In-vitro anti-inflammatory macrophages test. Depending on the formulation circumstances, the mean particle size of selected batch (F6) was 145.7±7.6 nm and 79.8 ± 6.1% encapsulation efficiency was recorded. The particle displayed smooth surface and no interaction between the excipients and drug. The in vitro release experiments demonstrated a quick burst release of the medication initially followed by a slow sustained release at different time interval of 24, 48, or 72 hours and proved good stability at both 25°C and 40°C. Also the nanoparticles inhibited hemolysis to a degree ranging from 3.8% to 11.1%. According to the study's findings, betamethasone and ketoprofen may be delivered as a sustained release manner using chitosan alginate nanoparticles.