Bifunctional reactive dyes were examined considering new reactive dyes fixation with wet fastness properties on to cotton fiber. The bi-functional reactive dyes along various groups 4- (β-sulfatoethylsulfonyl) aniline were studied on to cotton fabric with exhaust dyeing method to investigate the performance of groups on fixation of shades, tone and wet fastness behavior. The bi-functional reactive dyes synthesized by the coupling of diazotized 4-(βsulfatoethylsulfonyl) aniline by different cyanurated coupling components in good yield. These dyes were characterized by FTIR, 1 H NMR spectroscopic elemental analysis. Colorimetric data (L*, a*, b*, C*, H*, K/S) have been studied. The dye with various position of groups has individual affectability and affinity to the used cotton material. Synthesized bifunctional dyes performed good fixation at most favorable settings of temperature, pH and dyeing time were studied. Colorimetric data and K/S have also been investigated.