Various systems of medicine have been practiced worldwide for centuries, each with its unique principles, philosophy, and treatments. Objective: This study aims to develop, evaluate, and compare an antimicrobial herbal lotion using curcuma and Aloe vera extracts for dermatological applications. Materials and Methods: The importance of quality control in herbal medicine and the global preference for traditional medicines and herbal remedies are recognized. The formulation preparation involved the use of ethanolic extracts of Curcuma longa and Aloe vera roots, along with other ingredients. The lotion formulations were evaluated based on organoleptic properties, consistency, pH, spreadability, extrudability, viscosity, diffusion, hardness, loss on drying, solubility, washability, drug content, in vitro diffusion, ex vivo permeation, irritancy, and stability. Additionally, an in vivo experimental study using an excision wound model in Wistar albino rats was conducted to evaluate the prepared formulation. The study included group division, dose schedule, treatment period, and assessment of wound closure time, percentage of wound contraction, time of epithelialization, and histological studies. Results: The results of this study provide valuable insights into the development of an effective antimicrobial herbal lotion for dermatological applications.