Biochemical approaches play a crucial role in the process of drug discovery and development. These approaches encompass a wide range of techniques and methodologies that enable researchers to identify and optimize potential drug candidates with high efficacy and minimal side effects. This abstract provides an overview of the various biochemical approaches employed in drug discovery and development, including target identification and validation, high-throughput screening, structure-activity relationship studies, lead optimization, and preclinical testing. Additionally, it highlights the integration of computational tools and technologies in guiding and enhancing biochemical approaches. The utilization of these techniques not only accelerates the drug discovery process but also improves the chances of successful translation from bench to bedside. Overall, biochemical approaches form the foundation of modern drug discovery, enabling the development of innovative therapies for various diseases and medical conditions.