Depression is a prevalent mental illness. According to World Health Organization, 5% adults worldwide experience depression. The Bombax ceiba is a huge, beautiful, and thrive in the hot climate of India, even reaching altitudes of 1,500 meters. The current research emphasizes on the biochemical estimation for antioxidant and anti-depressant potential of hydroethanolic flowers extract of Bombax ceiba. Fresh flowers of B. ceiba were collected from the UP east region. Diethyl ether was obtained from the local market. Imipramine was obtained as gift sample from the Lupin Pharma Pvt Ltd. Evaluation kits for antioxidant were purchased from Thermofisher Scientific. The fresh flowers were harvested from the Uttarakhand region of India. The plant was authenticated by Botanist at NBRI Lucknow with specimen no. NBRI/PA/2022/10/0035. Wistar rats of either sex of weight b/w 40-60g were procured from the animal house at TRC Mahavidyalaya, Department of Pharmacy, Satrikh, Barabanki, India. The rats were divided in 4 groups and treated for 21 days i.e., control: administered normal saline, standard: administered Imipramine (10mg/kg, i. p.), test 1: administered Bombax ceiba flower extract (200mg/kg, p. o.), and test 2: administered Bombax ceiba flower extract (400mg/kg, p. o.). The chronic unpredictable mild stress (CUMS) was used for induction of depression. The evaluation parameters including body weight determinations, light-dark arena, sucrose preference test (SPT), biochemical evaluation. In results, Bombax ceiba facilitated the no. of entries and time spent in open arm in light/dark arena model and increased the sucrose preference percentage. Thus, it exhibited anti-depressant effect in behavioral and biochemical parameters i.e., SOD, CAT, TBARS and GSH. It can be concluded that B. ceiba hydroethanolic flowers extract dominates the inhibitory action of central nervous system. Its mode of action is required to be confirmed by screening in clinical trials to confirm for safety and efficacy.