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ISSN 2063-5346
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Biofilm detection among clinical isolates of Candida auris

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Festus Joseph.M, Abitha Evangelin, Dr. Priyadarshini Shanmugam, Dr. Ambujavalli Balakrishnan Thayikkannu
» doi: 10.31838/ecb/2023.12.6.264

Abstract

Candida auris is a yeast of concern in health care settings, due to its multi-drug resistant nature and outbreak potential. It survives on the patient’s skin as commensal and in the immediate surroundings for long durations, and its existence as biofilms alarms physicians and infection control experts in treating and containing this infection. Aim: To perform phenotypic tests to observe biofilm formation among clinical isolates of Candida auris. Methodology: Candida species were detected and isolated from various clinical samples by conventional, automated culture methods. Isolated Candida species were inoculated on Selective Auris Medium to prove as Candida auris and they were confirmed by MALDITOF MS and were assessed for biofilm formation using Congo red agar, tube method and microtiter plate method. OD values were calculated and mean values were taken. Results: Out of 459 isolates 20 were identified as Candida auris and included. 20 isolates confirmed by PCR RFLP from archives were also included along with ATCC C.albicans and C.parapsilosis as controls. 10 were biofilm producers by all three methods. Conclusion: This study shows that biofilms can be detected easily and detection can be done concurrently to plan effective treatment, isolation and prevention strategies and improve infection control practices.

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