Euphorbia hirta belongs to the Euphorbiaceae family and is highly valued for its medicinal properties. The plant's leaves are consumed as a vegetable by tribal communities, and it is widely used in traditional Indian medicine to treat diabetes. Nanotechnology is a field that focuses on producing nanoparticles of varying sizes, shapes, and chemical compositions to benefit human health. Plant-mediated biological synthesis of nanoparticles is becoming increasingly popular due to its simplicity and eco-friendliness. This present study involved using the leaf extract of Euphorbia hirta to carry out green synthesis of silver nanoparticles at room temperature. The nanoparticles were characterized using UV-Vis Spectrophotometer and FTIR, and their antimicrobial activity was evaluated on gram-positive (Staphylococcus aureus) and gram-negative (Escheria coli) bacteria. The plant's in vitro antidiabetic potential was determined using alpha amylase, alpha glucosidase, and DPP-IV enzyme inhibition study. The nanoparticles of E.hirta show a strong antidiabetic potential in an in vitro model, according to the findings of the current investigations, and more research can be done in an in vivo model.