Silver nanoparticles (Ag-NPs) have been synthesized using a variety of techniques, however because of their enormous size and little surface area, their catalytic activity has diminished. So, this article reports on the fungus-mediated synthesis of Ag-NPs. The generated particles were determined to be 23.6 nm when the synthesized Ag-NPs were further examined using XRD, SEM, EDS, and UV-Vis spectroscopy to evaluate the particle size, surface, crystalline nature, and phase formation of Ag-NPs. Ag-NPs are a fantastic class of broad-spectrum antibacterial agent. More noticeably, Aspergillus fumigatus' Ag-NPs may have powerful antibacterial activity against specific infections.