Sepsis is a serious condition that can lead to multiple organ failure and death. Blood lactate levels have been widely used to assess the severity of sepsis, but their predictive value in non-shock septic patients is uncertain. Objectives: The aim of this retrospective study was to investigate the association between blood lactate levels and mortality in non-shock septic patients. Material and Methodology: We analyzed the medical records of non-shock septic patients admitted to a hospital between 01 February 2021 to 30 June 2022. Blood lactate levels were measured on admission and at 24 hours after admission. The primary outcome was all-cause mortality within 28 days of admission. We used logistic regression models to assess the association between blood lactate levels and mortality, adjusting for potential confounders. Results: The study included 250 non-shock septic patients, with a mean age of 58±16 years and a 28-day mortality rate of 9.2%. Higher blood lactate levels on admission and at 24 hours after admission were significantly associated with increased mortality, with adjusted odds ratios of 5.377 (95% CI: 3.319-8.699, p < 0.001), respectively. Conclusions: Our study suggests that blood lactate levels can serve as a useful predictor of mortality in non-shock septic patients. Measurement of blood lactate levels on admission and at 24 hours after admission can help identify patients at high risk of death and guide clinical decision-making. Future studies are needed to validate these findings and investigate the potential benefits of interventions aimed at reducing blood lactate levels in non-shock septic patients.