Acne is a very common inflammatory disorder of the pilosebaceous unit that consists of comedones, inflammatory papules, pustules, and nodules involving the face, chest, and back. The pathogenesis of acne is complex and involves inflammation and release of cytokines around the pilosebaceous unit, abnormal keratinization, increased sebum production, and Propionibacterium acnes. An unfortunate sequela of acne is residual scarring and disfigurement. Acne and acne scarring can have a detrimental impact on the quality of life and lead to feelings of embarrassment and low selfesteem. Therapies for acne scarring included surgical modalities, such as subcision, and punch excision and elevation, injectable fillers, chemical peels, dermabrasion, microneedling, and energy-based devices. In the past decade, there has been a trend toward using cosmetic fillers and energy-based devices to improve acne scarring. Fat grafting has the advantages of wide source, convenient acquisition, good biocompatibility, and less surgical trauma, and was first applied in soft tissue filling, such as defect repair and deformity correction. In recent years, with the deepening of research, it has been found that stromal vascular fraction (SVF) cells and adipose-derived stem cells (ADSCs) in transplanted fat have multilineage differentiation ability, can differentiate into adipocytes, osteocytes, chondrocytes, and nerve cells in different environments, and can secrete cytokines such as vascular endothelial growth factor (VEGF), hematopoietic growth factor, and basic fibroblast growth factor (bFGF), which can effectively improve the survival rate of transplanted fat. Although nanofat does not contain mature adipocytes, it is rich in a large number of SVF, which contains different types of cells such as endothelial cells, granulocytes, monocytes, and macrophages, and also includes a large number of MSCs. ADSCs are MSCs found within the SVF of subcutaneous adipose tissue. ADSCs self-renew display a multilineage developmental plasticity used in various tissue repair and regeneration clinical studies, nanofat injection could be effective in improving the scar characteristics as well as symptoms and aiding in scar rejuvenation.