Nanotechnology is a rapidly growing research topic in all areas like engineering, medicine, pharmacy and science which involves materials with a size of 1–100 nm. Many researchers are now interested in obtaining metal and metal oxide nanoparticles via biological methods as eco-friendly and less toxic. Compared to physical and chemical synthesis, the green approach is found to be safer and more eco-friendly. Many sectors rely on zinc oxide for its varied qualities which have been strengthened through nanoscale manufacturing. Green production of zinc oxide nanoparticles utilizing several biological substrates has been proven and achieved. This study summarized recent breakthroughs in biosynthesized ZnO NPS synthesis, mechanism, characterization, and applications in agriculture, medicine, and other sectors.