Anredera cordifolia is a member of the Basellaceae family with great potential to be developed. Basellaceae contains antimicrobial and antioxidant properties that have been reported the secondary metabolites present in it include terpenoids, steroids, saponins, alkaloids, flavonoids, and tannins which make them useful for medicinal purposes. This study aimed to induce callus of A. cordifolia stems with growth regulator Benzyl Amino Purine (BAP) on Murashige and Skoog (MS) medium. The concentration of BAP used was 0 mg/L; 0.5 mg/L; 1.0 mg/L and 3.0 mg/L. The results obtained were able to produce callus formation. However, in control treatment, apart from forming callus, also forms leaves and roots. On 0.5 mg/L treatment, callus and leaves were formed. Callus was induced in the second week after planting. Callus textured crumbs and white. The highest average fresh and dry weight was the concentration of 1.0 mg/L BAP treatment.