Global warming as a result of the increase in greenhouse gases symbolizes a great threat to life on the planet, with the passage of time it has been reflected in the increase intemperatures causing major disasters and altering physical, biological and human systems, so it is sought mitigate its effects and preserve biodiversity. Therefore, the present research proposed to estimate the content of carbon sequestered in the natural grasslands of the Ichubamba Yasepan protected area, with the purpose of establishing the amount of carbon present in necro mass, biomass and soil stored in the high Andean natural grasslands to have real data for future negotiations of environmental compensations and thus contribute to the reduction of greenhouse gas emissions associated with deforestation and environmental degradation. In the grassland and shrub ecosystems 4 plots were established and sampling units of square shape with dimension of 10 x10 m were installed, two circular plots with a radius of 5 and 2.5 m were installed, 8 quadrants of 50 x 50 cm were formed according to the GLORIA methodology, where they were collected 18 necromass samples, 18 aerial biomass samples and 16 soil samples. These were processed in the laboratory of the Faculty of Natural Resources (FRN) of the Polytechnic School of Chimborazo (ESPOCh). For the statistical analysis, a database was built that was processed using the InfoStat statistical package,