In the realm of nanotechnology, there is a need for the creation of novel experimental procedures for the production of nanoparticles. The sol-gel process was used to synthesize Catechin-AgTitania nanoparticles. Catechin-Ag-Titania NPs is proposed as a novel cost-effective and ecofriendly antifouling compound. Well-dispersed Catechin-Ag-Titania NPs designated as CAT NP has been achieved through catechin mediated reduction of silver and TiO2 ions at ambient temperature. The CAT NP showed effective antifouling activity against the screened biofilm forming bacteria. SEM, XRD, FTIR and TEM showed the properties of CAT NP and antifouling properties of CAT NP will provide new opportunities to develop cost-effective and ecofriendly antifouling composition that is 10 times effective compared with control.