MS is a chronic immune-mediated disease of the central nervous system that often leads to long-term physical disability. Thus, the long-lasting chronic disease affects the mental health and quality of people who have MS. Many researches have been conducted on the topic of MS with various psychological factors and symptoms. Yet, despite the increase in the associations of symptoms and quality of life of MS patients, it has received considerable attention in characterizing pain. However, there was still little understanding of the association of pain with mental health quality of life. Therefore, this study was presented to explore the characterization of pain and mental health in patients with MS chronic disease. A sample has been gathered from 199 patients diagnosed with MS from the Neurology clinic in Bangalore. By using various statistical tools, the results of a study have been analyzed. The result showed that the pain experienced significantly more severe symptoms of depression and anxiety, which were significantly worse for the mental health quality of life of patients. Further, it showed that past pain reported the highest percentage than the current pain of MS patients.