Cinnamomum malabatrum has been traditionally used in India. The extracts of the leaves after column chromatography the compounds were analyzed to the LCMS/MS has led to the identification of active principles, among the peaks, 6 peaks were selected as per reference standard which furnished in the chromatograms. The constituents of Cinnamomum malabatrum are Gallic acid, Trans – Aconitic acid, Chlorogenic acid, Resorcinol, Rhanmetin, and Quercetin. Molecular docking analysis revealed that compound 1, 4, 5 has better docking efficiency and forms hydrophobic interactions with human beta tubulin This is suggests that the compounds act as potential inhibitors of polymerization of beta tubulin and formation of spindle network apparatus in the rapidly dividing cells during metaphase configuration. This is the best evidence for cell cycle specific anti cancer activity.