Towards exploring charge transfer behavior in polyaniline based composites design and study are alarming. This work is intended to study the charge mobilisation behavior by measuring the DC conductance of four different compositions of PANI-α-amino acids together with DFT, synthesis and physic-chemical studies. The composites are PANI-L-cysteine hydrochloride, PANI-L-serine and ES-L-tyrosine. Computational structural and TDDFT measurements were performed through B3LYP/6-31G** and PBE0/TZV.It was observed that the amino acids retard the charge mobilisation in the PANI and PANI-TYR has higher mechanical strength.