Using biodegradable chelators to assist phytoextraction may be an effective approach to enhance the heavy-metal remediation efficiencies of plants. A pot experiment was conducted to investigate the effects of various synthetic chelators and low molecular weight organic acids (LMWOAs) on the growth of hyperaccumulator guinea grass and its Chromium uptake and accumulation in tannery-contaminated soil. The addition of 5-mmol kg-1of Citric acid, (dose selected as per literatures) has a significant uptake of total and Hexavalent (VI) Chromium among the chelators at the end of the pot experiment (90th day) as 17.98 ppm and 2.94 ppm, respectively. Among the synthetic chelators, EDTA @ 5mmol/kg had resulted in the maximum uptake of Chromium as 2.42 mg/kg (hexavalent Cr) and 15.76 mg/kg (Total Cr), which indicates the best chelator next to citric acid. The phytoremediation technology can be best studied using chelators, which makes Chromium more bioavailable in Guinea grass, a hyperaccumulator plant.