Aim of the present investigation is designed to identify the phytoconstituents in ethanolic extract Cucurbita moschata and to assess the anti-epileptic potential of Cucurbita moschata leaf extract. One of the most frequent neurological illnesses is epilepsy. Cucurbita moschata has long been used to cure a variety of ailments. The present investigation was designed to identify the phytoconstituents in ethanolic extract Cucurbita moschata and to assess the anti-epileptic potential of Cucurbita moschata leaf extract. The National Institute of Standards and Technology database was used to interpret the mass spectrum of the GC-MS. The ethanolic extract was examined orally at different on experimentally induced animal. Phenytoin (40mg/kg), Valproic acid (200mg/kg) and Diazepam(10mg/kg) were used as the standard drugs for comparison. The parameters considered to assess the anti-epileptic activity were decrease in extensor phase, but there is no significance increase in onset of convulsion and decrease in the severity of status epilepticus as compared to the standard group. Significant anti-epileptic activity was showed by ethanolic suspension in MES model. Conclusively, the Cucurbita moschata leaf extract has potential to treat the grandmal epilepsy but there is no significant action against petitmal epilepsy and status epilepticus. GCMS analysis revealed presence of Pentacuclic terpenoid, Steroid acids and fatty acid esters. Antiepileptic activity may be attributed to pentacyclic triterpenoid, steroid and fatty acid esters.