The term ethnobotany means the study of the plants in a particular region and their uses based on indigenous cultural beliefs and practices by the local population. And such treatment is not related with modern physician drug. During the study it was observed that the traditional healer used many plants which are documented in local customs involving the practical uses of local flora for many aspects of life, such as, plants as medicines, food and clothing etc, by the different communities. In those regions still practiced and rely on ethno medicine at the basic level treatments. Manipur is one of the north east states having 34 major tribes and some smaller ethnic groups which belonging to various socio-cultural traditions and speak different languages. The present studies attempt to highlight the traditional knowledge of three different community related to the treatments of many ailments by different medicinal plants of Manipur. An attempt has been made to see the common medicinal plants species which are used for different purposes by different communities of Manipur. Due to over exploitation, modernization, urbanization, jhum cultivation, deforestation and construction of roads etc. are affecting medicinal plant diversity and such important traditional practices of medicinal plants is gradually losing. It is urgently required for conservation, scientific validation and recognition of traditional healing medicine and reactivation for the betterment of this neglected knowledge. There is immediate record of information for the benefit of mankind in future because the knowledge of medicine is transmitted orally and no written record was found. So, an attempt has been made to conserve and document such rich indigenous knowledge of ethno medicinal plants benefit used by different communities of Imphal East and Imphal West Districts of Manipur.