The elimination of microorganisms through chemomechanical preparation is essential for the success of endodontic treatment. To perform an effective pulpectomy in primary teeth, it is essential to use rotary files with customized taper, length and tip size which is suitable for morphology of root canal for primary teeth.The present study was conducted to assess centralization ability and canal transportation of root canals prepared with three commercially available pediatric rotary files Aim:To assess and compare the centralization ability and canal transportation of root canals prepared with three commercially available pediatric rotary files Materials & Methods:45 extracted human primary teeth with a minimum 7 mm root length were randomly divided into three groups. Each group comprised of 15 teeth. Group I - Kedo-SG blue, group II - Kedo-S square, and group III - Pro AF baby gold. Biomechanical preparation was done according to the manufacturer’s instructions. Pre- and post-instrumentation CBCT images were taken for each group to evaluate the remaining dentin thickness thereby assessing the centering ability and the canal transportation ability of different file systems. Results: The canal transportation value at 3 mm was 0.90, 0.91 and 0.92, at 5 mm was 0.92, 0.93 and 0.94 and at 7 mm was 0.94, 0.95 and 0.96 in group I, II and III respectively. A non- significant difference was observed in all the groups (P< 0.05). The canal centering ratio at 3mm was 0.07, 0.04 and 0.03, at 5 mm was 0.06, 0.03 and 0.01 and at 7 mm was 0.05, 0.01 and 0.01 in group I, II and III respectively. The difference was significant for all the values (P< 0.05). Conclusion: All three file systems tested in the study were effective in removing the radicular dentin. However, Kedo-SG Blue and Pro AF Baby Gold rotary file systems showed comparatively less canal transportation and more centering ability than Kedo-S Square rotary file system.