Aim: Present study compares the effectiveness of the Pro-Taper Universal retreatment System (PTR) using four different techniques for removing the AH Plus sealer. Methodology: Forty extracted mandibular premolar were decoronated to a standard size of 16 mm. Canals were prepared till Pro-Taper F2 size and obturation was done using Gutta percha and AH-Plus sealer. The specimens were arbitrary divided into four groups (n=10) each. Re-treatment was executed with, Group I - PTR system without solvent, Group II - PTR system with resin solvent, Group III - PTR system succeeded by Pro-Taper finishing files without resin solvent, Group IV- PTR system succeeded by Pro-Taper finishing files with resin solvent. All the teeth were sectioned and stereomicroscopic images were used for analysis with imageJ analyzer software. Results: None of the technique completely removed the root canal filling material. The PTR system succeeded by Pro-Taper finishing files along with resin solvent showed better outcomes. Conclusion: All the techniques were not able to remove the entire root canal filling material. The PTR system succeeded by Pro-Taper finishing files along with resin solvent showed efficient cleaning and showed lesser remnants.