Background: Colonization of teeth by Streptococcus mutans is one of the most important risk factors in the development of dental diseases. The dental plaque is a structure of vital significance as a contributing factor to at least the initiation of the carious lesion. S. mutans is considered to be the chief etiological agent in human dental caries. The reduction of sugar intake and its replacement with non-fermentable sweeteners is considered a useful approach to caries prevention. These sugar substitutes show anti-bacterial and anti-cariogenic activity at a stipulated quantity.