Apical extrusion of debris leads to postoperative inflammation and endodontic failure. Objective of the study is to comparatively evaluate debris extrusion by using Waveone rotary files, ProTaper rotary files. Material and Method: Forty Extracted single rooted human premolar teeth will be included in the study. After access opening, canal patency will be established by 15 K-file till the apical foramen followed by determination of working length by visual method. Now the samples will be divided into 2 groups: Group1: WaveOne, Group 2: ProTaper file. Following this the manufacturer's instructions will be followed for the instrumentation. During instrumentation, extruded debris and irrigants will be collected into the preweighed test tubes. Following this, these tubes will be stored in an incubator at the temperature of 70°C for next 5 days. To evaluate the resultant weight of extruded debris, these test tubes will be weighed. Result: Result of this study showed that Waveone was associated with more debris extrusion compared with ProTaper rotary file. Conclusion: All instrumentation techniques were associated with debris and irritant extrusion but Wave one rotary file produce significantly more debris extrusion compared to ProTaper files