To Compare and evaluate push out bond strength of Light cure MTA, BIO MTA+, Light cure Glass Ionomer in furcal Perforation with and without blood contamination in extracted mandibular 1st molar teeth. Methods and materials- In present study two groups were created based on contamination or non-contamination with blood, and further subdivided according to Light cure MTA, BIO MTA+, Light cure Glass Ionomer materials which were used. Perforations were filled with the test materials and placed in Humidifier at 37°C and 100% relative humidity for 7 day. Push out bond strength was calculated on Universal testing machine. Statistical analysis- Intra-group comparison was done using one way Anova test and inter-group comparison was done using unpaired t – test. Results- The lowest (5.02 MPa) bond strength value was recorded in group Light cure MTA with blood contamination, where as highest (23.31 MPa) bond strength value was recorded in group Light cure GIC without blood contamination. The group of BIO MTA+ showed intermediate values. Conclusion- Within its limitations, the study showed blood contamination decreased the Push out bond strength of materials and Light cure GIC has excellent Push out bond strength compared to other test materials.